Florida - Area of significance: Transportation
| Alligator Reef Light |
| Allison, George, House |
| Amelia Island Lighthouse |
| American Shoal Light |
| Anclote Key Lighthouse |
| Apopka Seaboard Air Line Railway Depot |
| Arcadia Historic District |
| Atlantic Coast Line Railroad Depot |
| Atlantic Coast Line Station |
| Atlantic Gas Station |
| Avon Park Historic District |
| Barracks and Mess Building-US Coast Guard Air Station at Dinner Key |
| Bay City Walking Dredge |
| Bellamy Bridge |
| Blackburn Point Bridge |
| Boca Grande Lighthouse |
| Bridge of Lions |
| Cape Canaveral Air Force Station |
| Carysfort Lighthouse |
| Cedar Keys Historic and Archaeological District |
| Charlotte Harbor and Northern Railway Depot |
| CITY OF HAWKINSVILLE (shipwreck) |
| Crawlerway |
| Curry Houses Historic District |
| Dade City Atlantic Coast Line Railroad Depot |
| Dixie Highway-Hastings, Espanola and Bunnell Road |
| Emanuel Point Shipwreck Site |
| Fernandina Beach Historic District |
| First American Road in Florida |
| Florida East Coast Railway Locomotive #153 |
| Florida Pioneer Museum |
| Florida State Road No. 1 |
| Fowey Rocks Light |
| GOVERNOR STONE (schooner) |
| Hialeah Seaboard Air Line Railway Station |
| High Springs Historic District |
| Homestead Town Hall |
| Jacksonville, Pensacola and Mobile Railroad Company Freight Depot |
| Jacksonville Terminal Complex |
| John Pennekamp Coral Reef State Park and Reserve |
| Jungle Trail |
| Jupiter Inlet Lighthouse |
| Key West Historic District |
| Lafayette Street Bridge |
| Lake Placid A. C. L. Railroad Depot, Old |
| Lake Wales Historic Residental District |
| Launch Complex 39 |
| Lloyd Railroad Depot |
| L & N Marine Terminal Building |
| Lock No. 1, North New River Canal |
| Longwood Historic District |
| Louisville and Nashville Depot |
| Louisville and Nashville Passenger Station and Express Building |
| MAPLE LEAF (Shipwreck Site) |
| McIntosh Historic District |
| McKenzie, Robert L., House |
| Melbourne Beach Pier |
| Meridian Road |
| Merrick-Simmons House |
| Michigan Avenue Bridge |
| Missile Crawler Transporter Facilities |
| Motor Inn Number 2 |
| Mount Dora A. C. L. Railroad Station, Old |
| Ocala Union Station |
| Old A.C.L. Union Depot |
| Old Gainesville Depot |
| Old Haulover Canal |
| Old Orlando Railroad Depot |
| Overseas Highway and Railway Bridges |
| Palatka North Historic District |
| Palatka South Historic District |
| Palmetto Historic District |
| Pan American Seaplane Base and Terminal Building |
| Pensacola Historic District |
| Pensacola Lighthouse and Keeper's Quarters |
| Pigeon Key Historic District |
| Pine Level Acheological District (8DE14) |
| Plant City Union Depot |
| Ponce De Leon Inlet Lightstation |
| Port Orange Florida East Coast Railway Freight Depot |
| Punta Gorda Atlantic Coast Line Depot |
| Sanibel Lighthouse and Keeper's Quarters |
| SAN PEDRO (shipwreck) |
| Seaboard Air Line Depot, Old-Sebring |
| Seaboard Air Line Dining Car-#6113 |
| Seaboard Air Line Lounge Car-6603 |
| Seaboard Airline Railroad Station |
| Seaboard Air Line Railway Station, Old |
| Seaboard Coast Line Railroad Depot |
| Seaboard Coastline Railroad Passenger Station |
| Shell Hammock Landing |
| Sombrero Key Light |
| Sopchoppy Depot |
| Spanish Fleet Survivors and Salvors Camp Site |
| SS COPENHAGEN (shipwreck) |
| SS Tarpon (Shipwreck) |
| Standard Oil Service Station |
| Star Garage |
| St. Augustine Lighthouse and Keeper's Quarters |
| St. Cloud Depot |
Data from United States Park Service and Wikidata
