| 12th Street Line | 1.6 |
| Aberdeen Subdivision | 267 |
| Albert Lea Subdivision | 136.3 |
| Bayard Subdivision | 97 |
| Blair Subdivision | 40.4 |
| Boone Subdivision | 121.6 |
| Cedar Rapids (CN) Subdivision | 42.1 |
| Cedar Rapids (IANR) Subdivision | 49.1 |
| Cedar Rapids Subdivision | 17.4 |
| Cherokee Subdivision | 103.8 |
| Chicago (CPRS) Subdivision | 102 |
| Chillicothe Subdivision | 230.2 |
| Clinton (UP) Subdivision | 201.4 |
| Council Bluffs (BNSF) Subdivision | 19.6 |
| Council Bluffs (IAIS) Subdivision | 133.4 |
| Creston Subdivision | 148.4 |
| Davenport Subdivision | 55.5 |
| Des Moines Subdivision | 63.5 |
| Des Moines Terminal | 7.5 |
| Dubuque Subdivision | 144 |
| Eldridge Subdivision | 9.5 |
| Estherville Subdivision | 79.4 |
| Fairmont Subdivision | 108.6 |
| First Division | 35.1 |
| Forest City Line | 29.7 |
| Fort Dodge Subdivision | 49.2 |
| Geneva Subdivision | 138.8 |
| Hannibal Subdivision | 218.5 |
| Ida Grove Subdivision | 24.3 |
| Iowa City Subdivision | 81.7 |
| Jewell Subdivision | 98.7 |
| Laredo Subdivision | 102.4 |
| Laurens Subdivision | 31 |
| Manly Subdivision | 67.4 |
| Marceline Subdivision | 209.3 |
| Marquette Subdivision | 160.5 |
| Marshall Subdivision | 223.1 |
| Mason City (CPRS) Subdivision | 116.8 |
| Mason City (UP) Subdivision | 121.9 |
| Napier Subdivision | 77.2 |
| Newton Subdivision | 96.5 |
| Omaha (CN) Subdivision | 131.7 |
| Omaha (UP) Subdivision | 65.6 |
| Osage Subdivision | 95.4 |
| Oskaloosa Subdivision | 67.7 |
| Ottumwa (BNSF) Subdivision | 210.6 |
| Ottumwa (CPRS) Subdivision | 108 |
| Owatonna Subdivision | 93.6 |
| Perry Subdivision | 8.4 |
| Prairie City Spur | 13.3 |
| Rake Subdivision | 57 |
| Rock Island Spur | 13.8 |
| Sheldon Subdivision | 137.1 |
| Sioux City (BNSF) Subdivision | 110.4 |
| Sioux City (UP) Subdivision | 69.4 |
| Tara Subdivision | 70.8 |
| Third Division | 3.9 |
| Trenton Subdivision | 181.6 |
| Waterloo Subdivision | 109.1 |
| Worthington Subdivision | 146.7 |
